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Apoptosis

Our apoptosis curriculum covers the basic understanding of what is happening in programmed cell death vs necrosis, and how to measure this using flow Cytometry as a tool.  Topics covered include

• Tunel Assays

• Caspases and how they relate to flow, and the assays which measure them, DEVD-Rhod110 and D2R for example

• Annexin V

• Mitochondrial DNA Probes such as Rhodamine123, CMXRos, JC-1, MitoTracker Green

• Toxins

• CFSE for cell growth/division and adding Hoechst for cell cycle in conjunction with CFSE to show halt in progression of cell cycle

• Hoechst dual emission shifts - red shift is an apoptotic indicator, as indicated by nuclear "blebbing" and DNA laddering

• And Multiplexing - using several assays such as Hoechst, annexin, CMXRos, for example to demonstrate apoptosis.

This course is offered usually in conjunction with other advanced courses.  Watch for it in several venues this fall.  Tuition for this course is $225. This course was developed by Andrew Beernink.