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References
Price Reduced 50% off for direct orders from Editors |
FloCyte has two possible programs for DNA studies, a Multiparameter DNA Cell Cycle Approaches Course, outlined below and the software training program for Modfit, from Verity. Both will help your group become efficient in DNA Cell Cycle analysis. Please contact FloCyte for more information on scheduling one or both of these courses.
Basics of Cell Cycle Analysis A very brief history of cell cycle Going from cell cycle to single parameter DNA histogram What do DNA histograms tell us – what other information is required for interpretation? Brief discussion of DNA dyes – do’s and don’ts Examples of perturbations in DNA histograms Sub-G1 cells – how to identify apoptotic cells from debris
Multiparameter Cell Cycle BrdU to more precisely determine S phase Traditional method New SBIP method Other assays for proliferation – Ki-67, cyclins Early two parameter approaches DNA versus protein DNA versus RNA Native versus Denatured DNA for identification of mitosis Use of cyclins to study cell cycle Background on role of cyclins, cdks and ckis Discussion of each of the useful cyclins: D, E, A and B Use of cyclins to study: Refinement of cell cycle compartments Drug treatment Unbalanced growth Drug point of action – stathmokinesis Difference between cancer and normal cells Three parameter approaches using cyclins and DNA Multiparameter approaches using modification of chromatin proteins Phosphorylation of histone H3 to identify mitotic cells Phosphorylation of histone H2AX to identify DNA double-strand breaks Sensitivity of the assay Detection of DNA damage by a variety of clastogens Measuring DNA damage using ATM Other multiparameter approaches Inactivated versus total retinoblastoma protein |
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