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References
Price Reduced 50% off for direct orders from Editors |
Multiparameter Flow Cytometry Our first course in Multiparameter Flow Cytometry, taught by Dr. Carleton Stewart, Director of the Flow Cytometry Lab of Roswell Park Cancer Center in Buffalo, New York, was a great success. We are following it with a curriculum build on Dr. Stewart's presentation on the basics of antibody binding kinetics, titering antibodies, extra and intracellular staining, using tandem dyes, blocking, viability and have augmented it with a complete compensation presentation, built on the work of Mario Roederer, Wayne Moore and Dave Parks. It is taught most often by Dr. Jennifer Wilshire, but others in our staff also present it, including Dr. Doug Swartzendruber, Carol Oxford, Marty Bigos, Peter Lopez and Rich Konz. The response to this course has been excellent! Registration is available at this website. Registrations will be accepted in the order they arrive, until the space is full. Curriculum covers these topics Introduction to the course Physics of light Fluorescence Fluorochromes and Reagents Considerations for selecting reagents Viability Controls Beads for Instruments and assays Isotypes vs No isotypes FMOs Compensation Controls (more later) Staining Methodology Titering and Saturation Issues Autofluorescence Problems Specific VS Non- specific fluorescence Compensation In Multicolor phenotyping How to do it correctly Measurement Error Hardware vs Software Bi-Exponential Scaling Compensation Controls Strategies for Building Multicolor Panels Antibody / Fluorochrome combinations Necessary, Important or Luxury Markers Calculating Spread and error Instrument configurations / filter choices Practice Designing Experiments Design Process Biological hypothesis
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