inverted CommasI can’t recommend FloCyte enough to people. I wish everyone who prepares flow samples – even if they don’t run them – would take FloCyte’s courses




 Open bookGood Methods Section

Doucett VP, Gerhard W, Owler K, Curry D, Brown L, Baumgarth N. Enumeration and characterization of virus-specific B cells by multicolor flow cytometry.J Immunol Methods. 2005 Aug;303(1-2):40-52.

Baumgarth N, Bigos M. Optimization of emission optics for multicolor flow cytometry. Methods Cell Biol. 2004;75:3-22.

Kantor AB, Roederer M   FACS analysis of leucocytes Weir’s Handbook of Experimental Immunology  1996   Chapter 49: 1-13


Holden T. Maecker,* Tom Frey, Laurel E. Nomura, and Joe Trotter Selecting Fluorochrome Conjugates for Maximum Sensitivity Cytometry Part A 62A:169 –173 (2004)   Also a BD White Paper

Perfetto SP, Chattopadhyay PK, Roederer M.  Seventeen-colour flow cytometry: unravelling the immune system. Nat Rev Immunol. 2004 Aug;4(8):648-55 Perfetto, S., DeRosa, S. and Roederer, M.

Polychromatic Flow Cytometry. In: Flow Cytometry,  Darzynkiewicz, Z., Tanke, H. and Roederer(ed.), Elsevier, San Diego,  (2004)

Perfetto, S. and Roederer, M.. Multicolor Flow Cytometry. In:  Cell Biology: A Laboratory Handbook. 3rd Edition., Celis, J. (ed.), Elsevier.  (2003)

Baumgarth, N. and Roederer, M.  A  Practical  Approach to multicolor flow cytometry for immunophenotyping.  J Immunological Methods 2000  243:77-97

Intracellular Cytokine Staining

Maecker  HT, Rinfret A, D’Souza P,  et al Standardization of cytokine flow cytometry  assays. BMC Immunol. 2005 Jun 24;6:13.

Roederer,  M., Brenchley, J. M., Betts, M. R. and De Rosa, S. C.  Flow cytometric analysis of vaccine responses: How many colors are enough? Clinical Immunology 2004  110:199-205

Bauer  KD, Jacobberger JW Analysis of Intracellular Proteins  Methods in Cell Biology  1994 41:351-76

Jacobberger JW  Stoichiometry of Immunocychemical Staining Reactions Methods in Cell Biology  2001 63: 271-98

 Standardization of Cytometers

NIH Vaccine Research Center website  Quality Control Procedures

 Hyperlog/Bi-Exponential Scaling

Bagwell CB. Hyperlog-a flexible log-like transform for negative, zero, and positive valued data.  Cytometry  A. 2005 Mar;64(1):34-42.

Tung JW, Parks DR, Moore WA, Herzenberg LA, Herzenberg LA. New approaches to fluorescence compensation and visualization of FACS data. Clin Immunol. 2004 Mar;110(3):277-83. Review.


Bagwell  CB, Adams EG.Fluorescence spectral overlap compensation for any number of flow cytometry parameters.Ann N Y Acad Sci. 1993  Mar 20;677:167-84. No abstract available. PMID: 8494206 [PubMed – indexed for MEDLINE]

Roederer M Spectral compensation for flow cytometry: visualization artifacts,             limitations, and caveats.Cytometry. 2001 Nov 1;45(3):194-205.

Data Presentation

Roederer M, Darzynkiewicz Z, Parks DR Guidelines for the presentation of flow cytometric data. Methods Cell Biol. 2004;75:241-56.

Darzynkiewicz, Z., Tanke, H. and Roederer, M (ed.), Data analysis and     presentation for Flow Cytometry. In: Flow Cytometry, Elsevier, San Diego.


Herzenberg, L. A. , Parks, D., Sahaf B., Perez, O. M Roederer, M., and Herzenberg, L.A.  The History and Future of the Fluorescence Activated Cell Sorter and Flow Cytometry: A View from Stanford.  Clinical Chemistry. 2002;48:1819-1827.