inverted CommasI can’t recommend FloCyte enough to people. I wish everyone who prepares flow samples – even if they don’t run them – would take FloCyte’s courses



Digital Imaging and Microscopy

Algae Spirogyra Focus Result 1 (B, 8, 4) basswood stem - Version 2 OLYMPUS DIGITAL CAMERA OBELIA LIVE1-23-2004_10-25-09_AM GLOMERULUS_DSP

Today’s technology in microscopy is an excellent addition to any flow cytometry lab and many of our labs are equipped with both!  We are now very happy to be able to offer an excellent curriculum on digital microscopy and imaging.   This course is taught by a foremost authority in the field, Dr. Brian Matsumoto!  We are delighted to add Digital Imaging to our curriculum.


  1. The Digital Image
    1. Definitions
    2. What is needed to resolve structure in digital imaging
      1. Nyquist Criteria
      2. High Signal to Noise
    3. Good microscopy technique to achieve maximum information
  2. Brightfield/Transmitted Illumination: Imaging Stained specimens
    1. Diffraction theory of resolution
      1. Role of Numerical Aperture.
      2. The rational of staining and sectioning
    2. Köhler Illumination
    3. The slide as an optical element
      1. Standardized thickness
      2. Role of coverglass/mountant
  3. Imaging Transparent Specimens
    1. Light and the specimen (phase effects)
    2. Phase contrast
    3. Differential Interference Contrast
    4. Darkfield
  4. Fluorescence Microscopy
    1. Fluorochrome
    2. Epi-illumination
    3. Fading, Quenching, Antifade
  5. Confocal Microscopy
    1. Advantages over other techniques
    2. Advantages of laser based system


  6. Digital Image Enhancement
    1. Cameras
    2. SNR revisited
    3. Image processing/adjustment
    4. The taking of good pictures, techniques