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inverted CommasI can’t recommend FloCyte enough to people. I wish everyone who prepares flow samples – even if they don’t run them – would take FloCyte’s courses

 

 

Fluorescent Proteins in Flow Cytometry

Curriculum for this 1/2 day advanced flow course covers these topics:

I.          Introduction to Fluorescent Proteinsglowingcat

  • ·         What are they,
  • ·         A little history, how they were discovered
  • ·         Why they are so useful.
  • ·         How they are made, and how they are used in experiments.
  • ·         Varieties currently available, and sources.
  • ·         Recommendations of FPs for use in flow.

II.          Expressing Fluorescent Proteins for use in flow and image cytometry

  • ·         Methods of transformation, transfection, and production of transgenotes.
  • ·         Laser and detection configurations.
  • ·         Compensation.
  • ·         New lasers.
  • ·         Subcellular targeting of FPs.
  • ·         Stability and toxicity issues.

 

III.         Practical Examples of Analysis of FP Expression using Flow and Image Cytometry

  • ·         Mammalian cells in culture.
  • ·         Plant cells and organs.
  • ·         Complex animal organs.
  • ·         Split FP reconstitution.
  • ·         The “Brainbow”.
  • ·         Kinetic FPs and nano-scale microscopy.
  • ·         Noise in gene expression