Curriculum for this 1/2 day advanced flow course covers these topics:
- · What are they,
- · A little history, how they were discovered
- · Why they are so useful.
- · How they are made, and how they are used in experiments.
- · Varieties currently available, and sources.
- · Recommendations of FPs for use in flow.
II. Expressing Fluorescent Proteins for use in flow and image cytometry
- · Methods of transformation, transfection, and production of transgenotes.
- · Laser and detection configurations.
- · Compensation.
- · New lasers.
- · Subcellular targeting of FPs.
- · Stability and toxicity issues.
III. Practical Examples of Analysis of FP Expression using Flow and Image Cytometry
- · Mammalian cells in culture.
- · Plant cells and organs.
- · Complex animal organs.
- · Split FP reconstitution.
- · The “Brainbow”.
- · Kinetic FPs and nano-scale microscopy.
- · Noise in gene expression