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inverted CommasI can’t recommend FloCyte enough to people. I wish everyone who prepares flow samples – even if they don’t run them – would take FloCyte’s courses

 

 

Introduction to Stem Cell Flow Cytometry Course

  • Introduction
    • What is a stem cell
      • Biology of the cells
      • Background of the research
        • Researchers –
          • Weissman, Crooks,
      • Uses / therapeutic and research
      • Rare Cell / rare events considerations in Sorting
      • statistics for good counting
    • Kinds of stem cells –
      • Human vs other species (Murine)
  • Embryonic vs Adult
  • Cancer
  • IPS
  • Stem Cell Protocols
  • In Flow Cytometry
    • Marking negative events – Dump channel contents
    • Marking Positive events
    • Hoechst 33342 and Py
    • Side Populations
    • Aldefluor
    • CDs
      • 34, 133, 38, 45, 10, 7, 24, 44,
  • GCTM2, TG10, OKT4, SSEA-4, SSEA-1
  • Plus….
  • Clinical enumeration
    • umbilical cord and mobilized peripheral blood
    • For Bone Marrow transplants?
    • CD34
  • Panel Design for different types of cells…
    • Hematopoetic
    • Mesenchymal
    • circulating cancer cells
  • Culture issues
    • Hypoxic
    • growth factors
  • Sorting issues
    • Rock Inhibitor
    • Viability
    • Pressure Issues / sorting rates vs High pressure
  • Other technologies
    • Stem Cell Technologies
    • StemStep
    • LEAP
    • Miltenyi kits