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inverted CommasI can’t recommend FloCyte enough to people. I wish everyone who prepares flow samples – even if they don’t run them – would take FloCyte’s courses

 

 

New Comprehensive 3 Day Course

Students can choose to register for any of 4 options for our 3 day comprehensive course curriculum:

Comprehensive Option: All 3 days of the course

Foundations of Flow Cytometry: 2 day basic option for novice users, days one and two

Principles of Flow Cytometry: 2 day intermediate option, days two and three

Practices of Flow:  one day option for Multiparameter experimental design Advanced Users only may register for day 3 only, please

 

Topics covered in our 3 day course:

Foundations of Flow Cytometry

Day 1

  • 1 Overview
  • 2 Fluorescence and fluorochromes including filters and the concept of overlap
  • 3 Basics of a flow cytometer (theory)
  • Walk through the cytometer
  • 4 What does data look like
  • 5 Using fluorochromes successfully
  • Bead experiment, live
  • 6 Applications

 

Day 2

Foundations of Flow Cytometry continues

Principles of Flow Cytometry Option begins here

  •  Review of Day 1 topics and overview of Day 2
  • 8 Signal detection and  processing
  • 9 Data analysis: Regions, gates and stats
  • 10 Compensation
  • Interactive session on Compensation
  • 10 Compensation (continued)
  • 11 Sample preparation
  • 12 Experimental optimization
  • Interactive session on Layouts; quizzes on basics and data
  • 13 Controls in cytometry

 

Graduation of our Foundation in Flow Cytometry Students

Day 3

Continuation of Principles of Flow Option

Practices of Flow Cytometry

  • 14 Review of Day 2 topics and overview of Day 3
  • 15 Experimental planning
  • Practical – simple experimental design,
  • 2 scenarios and rotate
  • 16 Experimental analysis
  • 17 Software
  • More complex experimental design (5 color)
  • 1 scenario
  • 18 Data presentation guidelines
  • 19 Rare event analysis
  • 20 Applications
  • Interactive analysis session (class)
  • “How to analyze”
  • 21 Other cytometric technology
  • 22 Summary, resources, wrap-up Q&A,

Graduation of Comprehensive, Principles of Flow, and Practices Students