Map
Testimonials

inverted CommasI can’t recommend FloCyte enough to people. I wish everyone who prepares flow samples – even if they don’t run them – would take FloCyte’s courses

 

 

BOOT CAMP course curriculum

bootcamp

Covering  the following topics in two very full days:

 

 

  • Introduction
  • Components of a flow cytometer –
    • fluidics, optics, electronics
  • Instrument Characterization
  • Instrument Demonstration
  • Applications
    • Variations on the Immunophenotyping
    • Intracellular Protein detection
    • Other special applications
    • Functional Assays
    • DNA analysis – Introduction
    • Molecular cytometry
    • End of Day 1
  • Designing Good Assays
    • Choosing Antibodies / Fluorochromes
    • Using Fluorochromes and Reagents
  • Staining and Controls
  • Compensation
  • How to do it correctly
    • Measurement Error
    • Hardware vs Software
    • Bi-Exponential Scaling
  • Introduction to  Flow Cytometric Data
  • how data is generated
    • linear/log, transforms, binning / channels
  • Data Display and Gating
    • Gating strategies –  live and post acquisition
    • Multicolor with emphasis on how to analyze and interpret
    • uni- and bi-variant histograms