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Testimonials

inverted CommasI can’t recommend FloCyte enough to people. I wish everyone who prepares flow samples – even if they don’t run them – would take FloCyte’s courses

 

 

Success in a Nutshell

This course will cover topics to help investigators understand how to achieve success in cytometry.  This will cover topics on the appropriate controls and how to use them; how calibration of instruments impacts experimental data; how to set appropriate voltages and understanding compensation, principles of data analysis, such as the use of FMO’s for setting gates, back gating and viability dyes.

Introduction to Cytometry

  • What it is and
  • how it works

Controls

  • Compensation
  • Calibration
  • Controls
    1. Experimental
    2. Instrumentation
    3. Reference

Data analysis and statistics

  • Gating
  • FMO controls
  • Statistics concepts
  • Statistical examples

Designing good assays

  • choosing Fluors
  • and Antibodies for best design